Most spectrophotometers can be controlled by a program provided by the manufacturer – an SCP (used here as an abbreviation for Spectrophotometer Control Program).
Generally, the SCP can be set to report data (e.g., single absorbance or fluorescence values) at a specified time interval and to save the resultant data to a file. By specifying the same reporting interval for both T-App and the SCP, and starting the two processes at the same time, the resulting data can be moved into a program such as Microsoft Excel to obtain absorbance or fluorescence as a function of temperature.
Most SCP programs can also be set to collect scans at a specified time interval and save the resultant scans to a file or files. By stepping or ramping the temperature, scans collected at a series of temperatures can be correlated with those temperatures.
The box below is an example procedure for manually using T-App in coordination with an SCP:
See Using Scripts with T-App for an example of a Script that would automate steps 3 – 10.