TLC 50™

The TLC 50™ is a temperature-controlled cuvette holder for fluorescence or other spectroscopic measurements where access to four sides of the cuvette is required.

Description
The TLC 50™ cuvette holder is similar to the TLC 40™ in construction. It consists of an insulated, temperature-controlled tower for a standard 10 x 10 mm square cuvette. To maximize thermal contact, the cuvette is held into one corner of the tower by a flexible metal clip. Four optical ports (10 mm wide by 12 mm tall) on each face of the tower permit the passage of the excitation and emission beams. The height of the optical center from the bottom of the cuvette is 8.5 mm to permit the use of standard microcuvettes requiring this dimension. A magnet and small motor below the cuvette permit magnetic stirring to minimize temperature gradients in the cuvette and to flush unwanted photoproducts from the illuminated volume.

The cuvette holder contains a thermoelectric cooler (Peltier device) for controlling the temperature. Circulating water (or other fluid) passes through a heat exchanger to draw off the heat generated by the cooler. A temperature probe is buried in one corner of the tower, even with the optical centerline next to two of the cuvette windows.

Dry gas may be directed through small holes in the optical ports next to the four cuvette windows to limit condensation when working at low temperatures. An opaque cover with an access cap can be placed over the tower and cuvette. An additional stream of dry gas purges the inside of the cap to limit access of moisture and ambient air to the top of the cuvette. The cover also limits stray light in the sample compartment.

Performance


Dimensions
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Sample Holder Temperature Ranges