ΔFlucs: Brighter Photinus pyralis firefly luciferases identified by surveying consecutive single amino acid deletion mutations in a thermostable variant

L. Halliwell, A. Jathoul, J. Bate, H. Worthy, J. Anderson, D. Jones, and J. Murray, Biotechnol. Bioeng. 115, 50-59 (2018)

“…Far‐UV circular dichroism (CD) spectra of Fluc proteins were acquired using 4 μm proteins analysed at 25°C in Applied Photophysics Chirascan (Surrey, UK) with a Quantum Northwest (Liberty Lake, WA) temperature controller…”